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Start Date

2023

Supporting Organization

Ankara University Scientific Research Projects Coordination Unit

Project Status

continues

Molecular and Morphological Characterization of Turkish Tardigrade Species and Utilization of Genome Data in Sustainable Agriculture and Medicine

Project Manager

Project Coordinator

Project Team

Emre Keskin , Işılay Çelik , Zeynep Ece İmamoğlu

Partners / Collaborations

Ankara University, Faculty of Science, Department of Biology

This study was planned to obtain the first genome-level data of the Tardigrada phylum, of which 65 species have been identified in Turkey to date but have been limited to morphological observations. The aim was to collect algae samples from designated locations between Antalya and Fethiye in the Mediterranean region, isolate the species in a laboratory environment, and determine the sequences of the species' protected regions and their entire genome sequences using different next-generation sequencing technologies.

Tardigrades, known as the most resilient animals to extreme conditions, have achieved widespread distribution across diverse geographies due to their survival strategies and resilience, which involve the temporary suspension of their active life. To date, 65 species have been identified in Türkiye, but these identifications have been limited to morphological observations. This planned study aims to address this deficiency by collecting algae samples from designated locations between Antalya and Fethiye (including Kaş-Kalkan) in the Mediterranean region. These samples will be brought to a laboratory for the isolation of the organisms. Morphological identification of the species will be carried out using Phase Contrast and Electron Scanning Microscopy, utilizing their morphological characteristics. First-generation sequencing of conserved regions of the identified species will be used, while whole genome sequences will be determined using second and third-generation sequencing technologies. This method, intended for comparative and complementary purposes, will contribute to the literature as the first genome-level sequence from Türkiye for the "Tardigrada" phylum.

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Ankara University, Faculty of Agriculture, Department of Fisheries Engineering, Subayevleri, 06120 Keçiören/Ankara

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